Forest Pathology Division, FRI, Dehradun, Uttarakhand *College of Horticulture & Forestry, Jhalawar, Rajasthan
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Diseased seedlings of Azadirachta indica were collected from Central Nursery, FRI, Dehradun and a pathogenic isolate (P) from neem seedlings was collected. The pathogen was isolated and identified as Acremonium species. So, vascular wilt in Azadirachta indica caused by Acremonium sp. is being reported for the first time from India in the present study. For testing pathogenicity, tests according to the Koch’s postulates were conducted. In the 4-monts net-house experiment, a total of 12 treatments were made in which AM fungus (Glomus fasciculatum), antagonistic fungi (Trichoderma viride and T. harzianum) and pathogen (Acremonium sp.) were treated singly as well as in combinations with each other. Results of the experiment showed the positive impact of Trichoderma spp. on germination and disease reduction in neem seedlings either singly or with AM and Trichoderma viride performed better than Trichoderma harzianum. Results also showed that AM was not effective to control disease when applied singly, but in combination with Trichoderma sp., it was found quite effective.
Keywords: Pathogenicity, Koch’s postulates, Azadirachta indica, Acremonium species, antagonists, arbuscular mycorrhizal fungus Glomus fasciculatum (AM), Trichoderma viride, Trichoderma harzianum,
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